Polymerase chain reaction for detection of Ureaplasma diversum from urogenital swabs in cattle in Australia

Smith, A., Chousalkar, K.K., and Chenoweth, P.C. (2012) Polymerase chain reaction for detection of Ureaplasma diversum from urogenital swabs in cattle in Australia. Australian Veterinary Journal, 90 (7). pp. 275-276.

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DOI: 10.1111/j.1751-0813.2012.00939.x

View at Publisher Website: http://dx.doi.org/10.1111/j.1751-0813.20...

Abstract

Background: Ureaplasma diversum has been associated with various reproductive problems in cattle, including granular vulvovaginitis, endometritis, salpingitis, early embryonic death, weak calves, decreased conception rates, balanoprosthitis, impaired spermatozoids and seminal vesiculitis in bulls.

Methods: This study briefly outlines the use of polymerase chain reaction (PCR) for the rapid detection of U. diversum directly from urogenital swabs collected from Australian beef cattle.

Results: The 16S ribosomal RNA gene sequences obtained from the PCR products of the clinical samples were closely related to U. diversum strain A417.

Conclusion: The present test enabled detection of the organism directly from clinical swabs collected from animals with or without lesions.


Item ID:	41115
Item Type:	Article (Short Note)
ISSN:	1751-0813
Keywords:	Australia, cattle, polymerase chain reaction, Ureaplasma diversum, urogenital swabs
Date Deposited:	07 Dec 2015 05:17
FoR Codes:	07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070206 Animal Reproduction @ 50% 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070707 Veterinary Microbiology (excl Virology) @ 50%
SEO Codes:	83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830301 Beef Cattle @ 50% 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830302 Dairy Cattle @ 50%
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