First complete genome of an Ambidensovirus; Cherax quadricarinatus densovirus, from freshwater crayfish Cherax quadricarinatus

Bochow, Shaun, Condon, Kelly, Elliman, Jennifer, and Owens, Leigh (2015) First complete genome of an Ambidensovirus; Cherax quadricarinatus densovirus, from freshwater crayfish Cherax quadricarinatus. Marine Genomics, 24 (3). pp. 305-312.

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Abstract

In 1999, the causative agent of an epizootic in Cherax quadricarinatus was described, and given the provisional name Cherax quadricarinatus parvovirus-like. Sequencing of the 6334 nt genome identified three open-reading frames on the top strand coding NS3 (35.55 kDa), NS1 (67.36 kDa) and NS2 (35.18 kDa) and on the bottom strand a single open reading frame which most likely encodes 4 structural proteins. Motifs characteristic of the Densovirinae were found in the ORFs. Phylogenetic analysis of the amino acids in NS1 places the genome in the genus Ambidensovirus, most closely related to the marine sea star densovirus (75%, E = 0.0) and distantly related to Acheta domestica densovirus (44.1%). The virus name is proposed as species Decapod ambidensovirus, variant Cherax quadricarinatus densovirus. This is the first Ambidensovirus to be found in decapod crustaceans and the first of the subfamily Densovirinae to be sequenced from a freshwater crayfish. Cherax quadricarinatus densovirus and sea star densovirus are the first highly related Densovirinae to infect phylogenetically disparate hosts and are thus far, unique among the Densovirinae.

Item ID: 40676
Item Type: Article (Refereed Research - C1)
Keywords: Cherax quadricarinatus; parvovirus; comparative genomics; genome annotation; phylogeny; densovirinae; ambidensovirus; aquaculture
ISSN: 1876-7478
Funders: Indo-Australia Biotechnology (I-AB)
Projects and Grants: I-AB Grant BF50090
Research Data: http://dx.doi.org/10.4225/28/57D8DCB5DF22B
Date Deposited: 29 Oct 2015 03:57
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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