Expressed genes in the lymphoid organ of broodstock banana prawn (Penaeus merguiensis) using suppression subtractive hybridization

Rusaini, , and Owens, Leigh (2015) Expressed genes in the lymphoid organ of broodstock banana prawn (Penaeus merguiensis) using suppression subtractive hybridization. Journal of Aquaculture & Marine Biology, 2 (1). 00016. pp. 1-9.

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Abstract

Most systemic viral infections in penaeid prawns are associated with the formation of lymphoid organ spheroid (LOS) cells in the lymphoid organ (LO). The development of spheroids is suggested to be a major defence mechanism against viral infection in penaeids. Spheroids were observed in a broodstock population of banana prawns (Penaeus merguiensis), while these changes were not identified in the wild population. This opportunity was taken to see how well suppression subtractive hybridization (SSH) would resolve a histological change with no known aetiology. A total of 316 sequenced clones were clustered into 141 contigs and 51.6% of the clones shared significant similarities to known peptide or nucleotide sequences in the NCBI GenBank database. These transcripts were assigned into 8 categories, including immune related genes (2.5%), synthesis, processing and regulation-related proteins (4.4%), ribosomal proteins (6.3%), proteases and inhibitors (7.0%), energy and metabolism factors (7.3%), structural and cytoskeletal related proteins (10.1%) and other sequences (13.9%). Many sequenced clones (48.4%) from the libraries had no significant similarity to amino acids/nucleotides in the public database revealing the ability of this method in disclosing new differentially expressed genes in the lymphoid organ of prawns. Despite the detection of differential gene expression in the SHH libraries, unfortunately there were no viral genomes identified which were associated with spheroid formation within the LO of penaeids. However, polymerase chain reaction (PCR) amplification with HPV140F and HPV140R primers suggested that the development of spheroid cells in the lymphoid organ of the broodstock population most likely due to Penaeus merguiensis hepandensovirus (PmeDV) which maybe the senior synonym of lymphoidal parvovirus (LPV).

Item ID: 40662
Item Type: Article (Research - C1)
ISSN: 2378-3184
Keywords: banana prawn; Penaeus merguiensis hepandensovirus; lymphoid organ; spheroid cells; suppression subtractive hybridisation; expressed genes
Additional Information:

Open Access by MedCrave Group is licensed under a Creative Commons Attribution 4.0 International License.

Funders: James Cook University
Date Deposited: 02 Oct 2015 02:53
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830105 Aquaculture Prawns @ 100%
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