Effect of CH₄/O₂ ratio on fatty acid profile and polyhydroxybutyrate content in a heterotrophic-methanotrophic consortium

Obulisamy, P. Karthikeyan, Chidambarampadmavathy, Karthigeyan, Nadarajan, Saravanan, Lee, Patrick K.H., and Heimann, Kirsten (2015) Effect of CH₄/O₂ ratio on fatty acid profile and polyhydroxybutyrate content in a heterotrophic-methanotrophic consortium. Chemosphere, 141. pp. 235-242.

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Abstract

Understanding the role of heterotrophic–methanotrophic (H–Meth) communities is important for improvement of methane (CH₄) oxidation capacities (MOC) particularly in conjunction with bio-product development in industrial bio-filters. Initially, a H–Meth consortium was established and enriched from marine sediments and characterized by next generation sequencing of the 16s rDNA gene. The enriched consortium was subjected to 10–50% CH₄ (i.e., 0.20–1.6 CH₄/O₂ ratios) to study the effects on MOCs, biomass growth, fatty acid profiles and biopolymer (e.g. polyhydroxybutyrate; PHB) content. Methylocystis, Methylophaga and Pseudoxanthomonas dominated the H–Meth consortium. Culture enrichment of the H–Meth consortium resulted in 15–20-folds higher MOC compared to seed sediments. Increasing CH₄ concentration (and decreased O₂ levels) yielded higher MOCs, but did not improve total fatty acid contents. PHB contents varied between 2.5% and 8.5% independently of CH₄/O₂ ratios. The results suggest that H–Meth consortia could potentially be used in industrial bio-filters for production of biopolymer/biofuel precursors from CH₄.

Item ID: 40648
Item Type: Article (Refereed Research - C1)
Keywords: greenhouse gas remediation, methane, methanotroph, next generation sequencing, bioproduct, biodegradable plastic
ISSN: 1879-1298
Funders: Advanced Manufacturing Cooperative Research Centre (AMCRC)
Projects and Grants: AMCRC grant number 2.3.4
Date Deposited: 02 Mar 2016 23:12
FoR Codes: 10 TECHNOLOGY > 1003 Industrial Biotechnology > 100305 Industrial Microbiology (incl Biofeedstocks) @ 40%
10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 40%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 20%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8298 Environmentally Sustainable Plant Production > 829802 Management of Greenhouse Gas Emissions from Plant Production @ 40%
82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8298 Environmentally Sustainable Plant Production > 829804 Management of Solid Waste from Plant Production @ 40%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 20%
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