Castillo, Cristina Cobo, Tanaka, Katsunori, Sato, Yo-Ichiro, Ishikawa, Ryuji, Bellina, Bérénice, Higham, Charles, Chang, Nigel, Mohanty, Rabi, Kajale, Mukund, and Fuller, Dorian (2016) Archaeogenetic study of prehistoric rice remains from Thailand and India: evidence of early japonica in South and Southeast Asia. Archaeological and Anthropological Sciences, 8 (3). pp. 523-543.

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Abstract

We report a successful extraction and sequencing of ancient DNA from carbonized rice grains (Oryza sativa) from six archaeological sites, including two from India and four from Thailand, ranging in age from ca. 2500 to 1500 BP. In total, 221 archaeological grains were processed by PCR amplification and primary-targeted fragments were sequenced for comparison with modern sequences generated from 112 modern rice populations, including crop and wild varieties. Our results include the genetic sequences from both the chloroplast and the nuclear genomes, based on four markers from the chloroplast and six from the nuclear genome. These markers allow differentiation of indica rice from japonica rice, the two major subspecies of Asian rice (O. sativa) considered to have separate geographical origins. One nuclear marker differentiates tropical and temperate forms of subspecies japonica. Other markers relate to phenotypic variation selected for under domestication, such as non-shattering, grain stickiness (waxy starch) and pericarp colour. Recovery and identification of sequences from nuclear markers was generally poor, whereas recovery of chloroplast sequences was successful, with at least one of four markers recovered in 61 % of archaeological grains. This allowed for successful differentiation of indica or japonica subspecies variety, with japonica identified in all the Thai material and a mixture of indica and japonica chloroplasts in the two Indian assemblages. Rice subspecies was also assessed through conventional archaeobotanical methods relying on grain metrics, based on measurements from 13 modern populations and 499 archaeological grains. Grain metrics also suggest a predominance of japonica-type grains in the Southeast Asian sites and a mixture of japonica and indica in the Indian sites with indica in the minority. The similar results of grain metrics and ancient DNA (aDNA) affirm that grain measurements have some degree of reliability in rice subspecies identification. The study also highlights the great potential of ancient DNA recovery from archaeological rice. The data generated in the present study adds support to the model of rice evolution that includes hybridization between japonica and proto-indica.

Item ID:	40367
Item Type:	Article (Research - C1)
ISSN:	1866-9565
Keywords:	archaeobotany; archaeogenetics; Ban Non Wat; Thailand; Prehistory; rice; prehistoric agriculture; Southeast Asia
Funders:	Research Institute for Humanity and Nature (RIHN), Hirosaki University (HU)
Projects and Grants:	RIHN Sato Project
Date Deposited:	26 Oct 2015 01:56
FoR Codes:	43 HISTORY, HERITAGE AND ARCHAEOLOGY > 4301 Archaeology > 430102 Archaeology of Asia, Africa and the Americas @ 60% 43 HISTORY, HERITAGE AND ARCHAEOLOGY > 4301 Archaeology > 430101 Archaeological science @ 40%
SEO Codes:	95 CULTURAL UNDERSTANDING > 9505 Understanding Past Societies > 950502 Understanding Asias Past @ 100%
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