Castro, David J., Maurer, Jochen, Hebbard, Lionel, and Oshima, Robert G. (2013) ROCK1 inhibition promotes the self-renewal of a novel mouse mammary cancer stem cell. Stem Cell, 31 (1). pp. 12-22.

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Abstract

The differentiation of stem-like tumor cells may contribute to the cellular heterogeneity of breast cancers. We report the propagation of highly enriched mouse mammary cancer stem cells that retain the potential to differentiate both in vivo and in culture and their use to identify chemical compounds that influence both self-renewal and differentiation. We identify epithelial tumor-initiating cells (ETICs) that express lineage markers of both basal and luminal mammary cell lineages and retain the potential, from even single cells, to generate heterogeneous tumors similar to the tumor of origin. ETICs can progress through a Rho-associated coiled-coil containing protein kinase 1 dependent, epithelial to mesenchymal transition to generate mesenchymal tumor-initiating cells capable of initiating tumors of limited heterogeneity. The propagation of ETICs may allow for the identification of new therapeutic compounds that may inhibit or prevent progression of some types of breast cancer.

Item ID:	40176
Item Type:	Article (Research - C1)
ISSN:	1945-4732
Keywords:	breast cancer; cancer stem cell; epithelial-mesenchymal transition; keratin; ROCK1
Funders:	Department of Defence Brest Cancer Research Program (DOD), USA , National Cancer Institute (NCI)
Projects and Grants:	NCI Cancer Centre Support Grant 5 P30 CA030199, NCI Postdoctoral Training Grant T32 CA121949
Date Deposited:	27 Aug 2015 03:48
FoR Codes:	11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111201 Cancer Cell Biology @ 100%
SEO Codes:	92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920102 Cancer and Related Disorders @ 100%
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