Wyder, Lorenza, Vitaliti, Alessandra, Schneider, Henriette, Hebbard, Lionel W., Moritz, Dirk R., Wittmer, Mariana, Ajmo, Malek, and Klemenz, Roman (2000) Increased expression of H/T-cadherin in tumor-penetrating blood vessels. Cancer research, 60. pp. 4682-4688.

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Abstract

Neovascularization is a prerequisite for tumor growth. Thus, selective destruction of the tumor vasculature should prevent tumor expansion. We have established a method to identify proteins that are specifically expressed on the surface of endothelial cells in tumors. CD31-positive endothelial cells were isolated from Lewis lung carcinoma lung metastases as well as from normal lung tissue. cDNAs derived from these cells were subjected to a subtractive hybridization procedure, and cDNAs overrepresented in tumor-derived endothelial cells were isolated; those encoding surface proteins were selected using a signal sequence trap assay. One isolated cDNA encoded H/T-cadherin. In this report, we show that mouse H/T-cadherin is overexpressed on endothelial cells of several tumors, whereas it is expressed only on a subset of endothelial cells in healthy organs. On the basis of the expression of H/T-cadherin in lung metastases of different tumors, we suggest that different tumors can have a differential influence on the expression of endothelial cell surface proteins.

Item ID:	40167
Item Type:	Article (Research - C1)
ISSN:	1538-7445
Funders:	Swiss National Foundation (SNF)
Projects and Grants:	SNF NFP37 Grant 40-44820.95
Date Deposited:	31 Aug 2015 02:20
FoR Codes:	06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060110 Receptors and Membrane Biology @ 100%
SEO Codes:	92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920102 Cancer and Related Disorders @ 100%
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