Identification of AGE-modified proteins in SH-SY5Y and OLN-93 cells

Langer, André K., Poon, H. Fai, Münch, Gerald, Lynn, Bert C., Arendt, Thomas, and Butterfield, D. Allan (2006) Identification of AGE-modified proteins in SH-SY5Y and OLN-93 cells. Neurotoxicity Research, 9 (4). pp. 255-268.

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The formation of “Advanced Glycation End products” (AGEs) is an inevitable consequence of mammalian glucose metabolism. AGE-mediated protein-protein crosslinks lead to detergent-insoluble and protease-resistant protein aggregates, and in Alzheimer’s disease (AD) extra cellular senile plaques (SPs) and intracellular neurofibrillary tangles (NFTs) have been shown to contain AGEs. However, to date little is known concerning the most prevalent protein-targets of AGE modification under normal, non-pathological conditions. Here, a combination of 2D-electrophoresis, Western blotting and mass spectrometry has been used to identify preferentially AGE-modified proteins in oligodendrocyte (OLN-93) and neuroblastoma cell lines (SH-SY5Y) in standard culture. Proteomics analysis identified a total of eight targets with structural, metabolic and regulatory function, three of which (ß-actin, ß-tubulin and eukaryotic Elongation Factor 1-α) were common to both cell lines. Based on results from prior studies, modification of these proteins may lead to a loss of function. Consequently, the identification of targets for these proteins is of particular interest for a better understanding of the consequences of AGE-modification in aging, neurodegenerative diseases and diabetes.

Item ID: 3937
Item Type: Article (Research - C1)
ISSN: 1029-8428
Keywords: AGEs; advanced glycation end products; Alzheimer's disease; diabetes; SH-SY5Y cells; OLN-93 cells; ß-abulin; Eukaryotic elongation factor 1-α; Aging; Neurodeco generative diseasctin - ß-tue - Diabetes
Date Deposited: 19 Nov 2009 23:38
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1109 Neurosciences > 110999 Neurosciences not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920111 Nervous System and Disorders @ 100%
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