First use of the WAVE™ disposable rocking bioreactor for enhanced bioproduct synthesis by N2-fixing cyanobacteria

Cirés, Samuel, Alvarez-Roa, Carlos, and Heimann, Kirsten (2015) First use of the WAVE™ disposable rocking bioreactor for enhanced bioproduct synthesis by N2-fixing cyanobacteria. Biotechnology and Bioengineering, 112 (3). pp. 621-626.

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Abstract

WAVE™ rocking disposable bioreactors have been successfully utilized for bioproduct development from bacteria, yeast, microalgae, and animal and plant cells but not from cyanobacteria so far. N2-fixing cyanobacteria represent a prolific bioproducts source with reduced cultivation costs. In this study, 1 L cultures of the N2-fixing cyanobacterium Anabaena siamensis grown diazotrophically in the WAVE™ bioreactor exhibited increased phosphate consumption and 37–70% higher CO2 fixation rates than those grown in conventional bubbled suspension (BS) batch cultures. This generated 40–80% increased biomass productivities in the WAVE™ bioreactor reaching 60 mg L−1 day−1 when supplemented with 10% CO2. Consequently, WAVE™ generated 36–153% more protein, lipid, and carbohydrate than BS, including 47–100% increased productivity of phycocyanin and stearidonic acid (SA) with relevant biomedical applications. While the type of culture system (BS or WAVE™) did not affect the biochemical profile of cyanobacterial biomass, 10% CO2 supplementation induced a significant decrease in fatty acids and phycocyanin contents (mg g−1 DW). Therefore, for commercial applications, the CO2 supplementation of WAVE™ should be optimized for each targeted bioproduct separately. This study opens possibilities for upgrading the WAVE™ systems to photobioreactors (PBRs) for bioproduct development from cyanobacteria, with opportunities and challenges critically evaluated herein.

Item ID: 36858
Item Type: Article (Refereed Research - C1)
Keywords: wave-mixed bioreactors; rocking bioreactors; cyanobacteria; phycocyanin; fatty acids; stearidonic acid
ISSN: 1097-0290
Funders: Australian Government’s Cooperative Research Scheme
Projects and Grants: Australian Government’s Cooperative Research Scheme, grant number 2.3.4
Date Deposited: 17 Jun 2015 00:29
FoR Codes: 10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 100%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8298 Environmentally Sustainable Plant Production > 829899 Environmentally Sustainable Plant Production not elsewhere classified @ 50%
97 EXPANDING KNOWLEDGE > 970105 Expanding Knowledge in the Environmental Sciences @ 50%
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