Efficient measurement of opsonising antibodies to Plasmodium falciparum merozoites

Hill, Danika L., Eriksson, Emily M., Carmagnac, Amandine B., Wilson, Danny W., Cowman, Alan F., Hansen, Diana S., and Schofield, Louis (2012) Efficient measurement of opsonising antibodies to Plasmodium falciparum merozoites. PLoS ONE, 7 (12). e51692. pp. 1-10.

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Abstract

Background: Antibodies targeting merozoites are important in protection from malaria. Therefore, merozoite surface proteins are attractive vaccine candidates. There is a need for robust functional assays to investigate mechanisms of acquired immunity and vaccine efficacy. To date, the study of merozoite phagocytosis has been confounded by the complexity and variability of in vitro assays.

Methodology/Principal findings: We have developed a new flow cytometry-based merozoite phagocytosis assay. An optimized merozoite preparation technique produced high yields of merozoites separated from haemozoin. Phagocytosis by the undifferentiated THP-1 monocytic cell line was mediated only by Fc Receptors, and was therefore ideal for studying opsonising antibody responses. The assay showed robust phagocytosis with highly diluted immune sera and strong inter-assay correlation. The assay effectively measured differences in opsonisation-dependent phagocytosis among individuals.

Conclusions/Significance: This highly reproducible assay has potential applications in assessing the role of opsonic phagocytosis in naturally acquired immunity and vaccine trials.

Item ID: 35855
Item Type: Article (Research - C1)
ISSN: 1932-6203
Additional Information:

© 2012 Hill et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: Victorian State Government, National Health and Medical Research Council (NHMRC), Institute of Health, US (NIH)
Projects and Grants: NHMRC Grant 1031212, NIH AI08968 6, NHMRC APP103571
Date Deposited: 31 Oct 2014 02:32
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 100%
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