Opsonising antibodies to P. falciparum Merozoites associated with immunity to clinical malaria

Hill, Danika L., Eriksson, Emily M., Li Wai Suen, Connie S.N., Chiu, Chris Y., Ryg-Cornejo, Victoria, Robinson, Leanne J., Siba, Peter M., Mueller, Ivo, Hansen, Diana S., and Schofield, Louis (2013) Opsonising antibodies to P. falciparum Merozoites associated with immunity to clinical malaria. PLoS ONE, 8 (9). e74627. pp. 1-8.

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Abstract

Naturally acquired humoral immunity to the malarial parasite Plasmodium falciparum can protect against disease, although the precise mechanisms remain unclear. Although antibody levels can be measured by ELISA, few studies have investigated functional antibody assays in relation to clinical outcomes. In this study we applied a recently developed functional assay of antibody-mediated opsonisation of merozoites, to plasma samples from a longitudinal cohort study conducted in a malaria endemic region of Papua New Guinea (PNG). Phagocytic activity was quantified by flow cytometry using a standardized and high-throughput protocol, and was subsequently evaluated for association with protection from clinical malaria and high-density parasitemia. Opsonising antibody responses were found to: i) increase with age, ii) be enhanced by concurrent infection, and iii) correlate with protection from clinical episodes and high-density parasitemia. Stronger protective associations were observed in individuals with no detectable parasitemia at baseline. This study presents the first evidence for merozoite phagocytosis as a correlate of acquired immunity and clinical protection against P. falciparum malaria.

Item ID: 35853
Item Type: Article (Research - C1)
ISSN: 1932-6203
Additional Information:

© 2013 Hill et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: Victorian State Government, National Health and Medical Research Council of Australia (NHMRC), National Institute of Health (NIH)
Projects and Grants: NHMRC Grant 1031212, NHMRC Grant 637406, NIH Grant AI089686
Date Deposited: 31 Oct 2014 02:38
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 100%
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