Modulation of C-reactive protein and tumour necrosis factor-alpha in collagen-induced arthritis in Dark Agouti rats: Impact of collagen concentration on severity of arthritis

Tudave, Dulanthi, Radhakrishnan, Ammu, Chakravarthi, Srikumar, and Haleagrahara, Nagaraja (2011) Modulation of C-reactive protein and tumour necrosis factor-alpha in collagen-induced arthritis in Dark Agouti rats: Impact of collagen concentration on severity of arthritis. Inflammation Research , 60 (10). pp. 897-907.

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Abstract

Objectives: The study investigated the effect of collagen-induced arthritis in Dark Agouti (DA) rats on the level of C-reactive protein and inflammatory cytokine tumour necrosis factor-alpha (TNF-α).

Subjects: Female Dark Agouti (DA) rats.

Methods: Three different dosages of (2 mg/kg of body weight, 3 mg/kg of body weight and 4 mg/kg of body weight) collagen and complete Freund’s adjuvant suspension were tested. After 45 days, serum C-reactive protein, TNF-α, superoxide dismutase and total glutathione assays were done. Radiographic and histopathological changes in the joints were compared.

Results: All three groups showed signs of arthritic changes, confirmed by histopathological and radiographic changes. Severe arthritic changes were seen in the rats injected with 4 mg/kg of body weight of collagen. There was a significant increase in C-reactive protein, TNF-α, super oxide dismutase and total glutathione levels in the plasma in arthritis rats and the changes were more significant with 4 mg/kg of collagen.

Conclusion: These results demonstrated that the optimal dose to inject to experimental animals in order to get server arthritic changes was 4 mg/kg of collagen with complete Freund’s adjuvant suspension. Severe arthritis changes induced significant elevation in plasma C-reactive protein and TNF-α levels.

Item ID: 35722
Item Type: Article (Research - C1)
ISSN: 1420-908X
Keywords: C reactive protein; collagen; Freund adjuvant; glutathione; superoxide dismutase; tumor necrosis factor alpha, animal experiment; animal model; animal tissue; article; body weight; concentration (parameters); controlled study; dark agouti rat; disease severity; experimental animal; female; histopathology; joint radiography; nonhuman; protein blood level; rat; rheumatoid arthritis
Date Deposited: 27 Oct 2014 23:31
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110322 Rheumatology and Arthritis @ 60%
11 MEDICAL AND HEALTH SCIENCES > 1116 Medical Physiology > 111603 Systems Physiology @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology @ 20%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920116 Skeletal System and Disorders (incl. Arthritis) @ 75%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 25%
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