Effects of cooling time on membrane integrity and motility of frozen-thawed canine spermatozoa using two different commercial egg yolk–based extenders at two different cooldown equilibration times

du Bois, S., Len, J.A., Parlevliet, J.M., and Eilts, B.E. (2012) Effects of cooling time on membrane integrity and motility of frozen-thawed canine spermatozoa using two different commercial egg yolk–based extenders at two different cooldown equilibration times. Reproduction in Domestic Animals, 47 (Supplement s6). pp. 278-280.

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Abstract

Two commercially available egg yolk–based semen extenders, one marketed for human semen freezing (HEYE) and one marketed for canine semen freezing (CEYE), were used to cryopreserve semen from single ejaculates of 11 different dogs. For each extender, a 30- and a 60–min cooldown period was used prior to the addition of the extender containing glycerol and then immediately frozen in liquid nitrogen vapours. Sperm motility was measured using a computer-assisted semen analysis (CASA) system. Sperm intact membranes were measured using SYBER-14 and propidium iodide. Semen in the HEYE cooled for 60 min had a significantly greater percentage of intact membranes than the semen in the HEYE cooled for 30 min (p = 0.02). Semen in the HEYE cooled for 60 min had significantly greater total motility (p = 0.007) and progressive motility (p = 0.004) than semen cooled for 60 min in the CEYE and semen cooled for 30 min in the HEYE (total motility p = 0.02 and progressive motility p = 0.02). Semen cooled for 60 min in the CEYE did not differ significantly in total (p = 0.6) or progressive motility (p = 0.4) than semen cooled for 30 min in the CEYE. There was no difference in total (p = 0.8) or progressive motility (p = 0.8) between the semen cooled for 30 min in the HEYE and the semen cooled for 30 min in the CEYE.

Item ID: 35587
Item Type: Article (Refereed Research - C1)
ISSN: 1439-0531
Date Deposited: 01 Oct 2014 15:52
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070206 Animal Reproduction @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830306 Horses @ 100%
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