Evaluation and optimization of multiple fluorophore analysis of a Pseudomonas aeruginosa biofilm

Baird, Fiona, Wadsworth, Marilyn P., and Hill, Jane E, (2012) Evaluation and optimization of multiple fluorophore analysis of a Pseudomonas aeruginosa biofilm. Journal of Microbiological Methods, 90 (3). pp. 192-196.

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Abstract

Conventional laser scanning microscopy for multiple fluorescent stains can be a useful tool if the problems of autofluorescence and cross-talk are eliminated. The technique of spectral imaging was employed to unmix five different fluorophores – ranging in emission from 435 to 665 nm – applied to a Pseudomonas aeruginosa biofilm with overlapping spectra and which was not possible using traditional channel mode operation. Using lambda scanning and linear unmixing, the five fluorophores could be distinguished with regions of differentiation apparent.

Item ID: 34416
Item Type: Article (Research - C1)
ISSN: 1872-8359
Keywords: biofilm; spectral imaging; Pseudomonas aeruginosa
Funders: NASA, NIH
Projects and Grants: NASA (NNX09AO60A), NIH (P20 RR021905-0)
Date Deposited: 11 Sep 2014 06:40
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110802 Medical Infection Agents (incl Prions) @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 50%
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