A PCR technique for forensic species level identification of abalone tissue

Sweijd, Neville A., Bowie, Rauri C.K., Lopata, Andreas L., Marinaki, Anthony M., Harley, Eric H., and Cook, Peter A. (1998) A PCR technique for forensic species level identification of abalone tissue. Journal of Shellfish Research, 17 (3). pp. 889-895.

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Abstract

Athough the incidence of abalone poaching is increasing in South Africa, several alleged poachers have been acquitted in cases where the state has been unable to prove that the confiscated meat is of the local abalone. Halimis midae. This species is illegally exported to the Far East by poaching syndicates, a practice that is undermining the legitimate industry. To solve this, a polymerase chain reaction (PCR) technique that targets a portion of the lysin gene of several abalone species and unequivocally distinguishes between H. midae and H. spadicea (a sympatric congeneric) has been developed. The PCR primers specifically amplify approximately 1 ,300 bp of genomic DNA from dried, cooked, and fresh abalone tissue. A smaller fragment of 1 46 bp is used for canned abalone. Restriction fragment length polymorphism (RFLP) exploit interspecific polymorphisms that discriminate between these two species. The method can also be used to identify H. rubra and can easily be adapted to other abalone species under the same threat of overexploitation.

Item ID: 34371
Item Type: Article (Research - C1)
ISSN: 1943-6319
Keywords: polymerase chain reaction (PCR); restriction fragment length polymorphism (RFLP);forensic; abalone; poaching; lysin
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Funders: Abalone Packers Association of South Africa, Foundation for Research Development
Date Deposited: 12 Aug 2014 03:04
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110701 Allergy @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics) @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
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