Id2 acts downstream of BMP signaling in chondrogenesis

Huang, Boyen, Takahashi, Katsu, Sakata-Goto, Tomoko, Kiso, Honoka, Tsukamoto, Hiroko, and Bessho, Kazuhisa (2011) Id2 acts downstream of BMP signaling in chondrogenesis. In: Abstracts from the 18th Annual Meeting of Bone Morphogenetic Protein for Medical Staffs. From: 18th Annual Meeting of Bone Morphogenetic Protein for Medical Staffs, 31 July 2011, Osaka, Japan.

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Abstract

Objectives: This study aimed to conduct a case-control investigation in maxilofacial morphogenesis, using a sample of Id2 homozygous knockout (KO) and wild-type (WT) mice. A special interest was to establish a relationship among Id2, BMP signals and chondrogenesis.

Materials and Methods: Appropriate ethics approval has been received. Crania collected from mice at the age of 0 day, 2 weeks and 12 weeks were assessed with a Euclidean Distance Matrix Analysis method. Cultured synchondroses of the murine cranial base were inspected with a histological approach and examined with a reverse transcription polymerase chain reaction technique.

Results: A shorter nasofrontal profile was seen in Id2 KO 12-week-olds but not 0-day-olds. The WT-KO gap in the skull length instead of the width increased with age. KO 2-week-olds showed a narrower hypertrophic zone and an inhibited proliferative zone in the presphenoid and the spheno-occipital synchondroses. Id2 expression in WT synchondroses was identified. Distribution of Type X collagen other than osteopontin was downregulated in Id2 KO samples. The WT murine cranial base showed a wider hypertrophic zone, a higher degree of ectopic hypertrophy and a larger number of proliferative chondrocytes in the presence of exogenous BMP2, BMP4 and BMP7, respectively. Such acquired growth was not detected in KO subjects. A 5-fold upregulation of Smad7 transcripts and a decrease in phosphorylation of Smad1-Smad5-and-Smad8-positive cells were identified in Id2 KO cartilage.

Conclusions: Postnatal chondrogenesis was related to Id2 that acted downstream to enhance BMP signals by inhibiting Smad7 expression.

Item ID: 34269
Item Type: Conference Item (Abstract / Summary)
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Date Deposited: 05 Aug 2014 04:10
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry > 110504 Oral and Maxillofacial Surgery @ 30%
11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry > 110507 Paedodontics @ 20%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060403 Developmental Genetics (incl Sex Determination) @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920113 Oro-Dental Disorders @ 30%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920116 Skeletal System and Disorders (incl. Arthritis) @ 70%
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