In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni

Pearson, Mark S., McManus, Donald P., Smyth, Danielle J., Lewis, Fred A., and Loukas, Alex (2005) In vitro and in silico analysis of signal peptides from the human blood fluke, Schistosoma mansoni. FEMS Immunology and Medical Microbiology, 45 (2). pp. 201-11.

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Proteins secreted by and anchored on the surfaces of parasites are in intimate contact with host tissues. The transcriptome of infective cercariae of the blood fluke, Schistosoma mansoni, was screened using signal sequence trap to isolate cDNAs encoding predicted proteins with an N-terminal signal peptide. Twenty cDNA fragments were identified, most of which contained predicted signal peptides or transmembrane regions, including a novel putative seven-transmembrane receptor and a membrane-associated mitogen-activated protein kinase. The developmental expression pattern within different life-cycle stages ranged from ubiquitous to a transcript that was highly upregulated in the cercaria. A bioinformatics-based comparison of 100 signal peptides from each of schistosomes, humans, a parasitic nematode and Escherichia coli showed that differences in the sequence composition of signal peptides, notably the residues flanking the predicted cleavage site, might account for the negative bias exhibited in the processing of schistosome signal peptides in mammalian cells.

Item ID: 34155
Item Type: Article (Research - C1)
ISSN: 0928-8244
Keywords: Schistosoma mansoni; signal peptide; signal sequence trap; parasite; transmembrane
Funders: National Health and Medical Research Council of Australia (NHMRC), University of Queensland (UQ)
Projects and Grants: NHMRC R. Douglas Wright Career Development Award
Date Deposited: 22 Sep 2016 06:03
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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