Identification of membrane-bound and secreted proteins from Echinococcus granulosus by signal sequence trap

Rosenzvit, Mara C., Zhang, Wenbao, Motazedian, Hossein, Smyth, Danielle, Pearson, Mark, Loukas, Alex, Jones, Malcolm K., and McManus, Donald P. (2006) Identification of membrane-bound and secreted proteins from Echinococcus granulosus by signal sequence trap. International Journal for Parasitology, 36 (1). pp. 123-130.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website:


The signal sequence trap technique was applied to identify genes coding for secreted and membrane bound proteins from Echinococcus granulosus, the etiologic agent of cystic hydatid disease. An E. granulosus protoscolex cDNA library was constructed in the AP-PST vector such that randomly primed cDNAs were fused with a placental alkaline phosphatase reporter gene lacking its endogenous signal peptide. E. granulosus cDNAs encoding a functional signal peptide were selected by their ability to rescue secretion of alkaline phosphatase by COS-7 cells that had been transfected with the cDNA library. Eighteen positive clones were identified and sequenced. Their deduced amino acid sequences showed significant similarity with amino acid transporters, Krebs cycle intermediates transporters, presenilins and vacuolar protein sorter proteins. Other cDNAs encoded secreted proteins without homologues. Three sequences were transcribed antisense to E. granulosus expressed sequence tags. All the mRNAs were expressed in protoscoleces and adult worms, but some of them were not found in oncospheres. The putative E. granulosus secreted and membrane bound proteins identified are likely to play important roles in the metabolism, development and survival in the host and represent potential targets for diagnosis, drugs and vaccines against E. granulosus.

Item ID: 34154
Item Type: Article (Research - C1)
ISSN: 1879-0135
Keywords: signal sequence trap; Echinococcus granulosus; secreted proteins; membrane bound proteins; developmental expression
Funders: Australian Centre for International and Tropical Health and Nutrition (ACITHN), National Health and Medical Research Council of Australia (NHMRC), Centro Argentino-Brasileño de Biotecnología (CABBIO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Date Deposited: 22 Sep 2016 05:48
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
Downloads: Total: 2
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page