Pearson, M.S, McManus, D.P., Smyth, D.J., Jones, M.K., Sykes, A.M., and Loukas, A. (2007) Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni. Parasitology, 134 (14). pp. 2001-2008.

PDF (Published Version) - Published Version Restricted to Repository staff only

 

3

8

Abstract

A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor - a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.

Actions (Repository Staff Only)

Item Control Page