Serum EBV DNA as a biomarker in primary nasopharyngeal carcinoma of Indian origin

Krishna, Smriti M., James, Susan, Kattoor, Jayasree, and Balaram, Prabha (2004) Serum EBV DNA as a biomarker in primary nasopharyngeal carcinoma of Indian origin. Japanese Journal of Clinical Oncology, 34 (6). pp. 307-311.

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Abstract

Background: Nasopharyngeal carcinoma (NPC) is a unique tumor due to its etiology and endemic distribution. Ethnic and regional factors are found to strongly influence the risk of disease; however, there have been no well-conducted studies on Indian patients. The present study assesses the relationship between Epstein-Barr Virus (EBV) and sporadic Indian NPC and the role of serum EBV DNA in NPC detection.

Methods: Primers directed against non-polymorphic Epstein-Barr nuclear antigen-1 (EBNA-1) gene were used to detect the presence of EBV DNA from fresh tissue and serum in NPC, using PCR.

Results: EBV DNA was detected in 69% of the biopsies and 58% of the serum of the NPC patients. With respect to histology, WHO Type III NPC, WHO Type II tumors and WHO I tumors showed 100%, 72.2% and 33% EBV positivity, respectively. EBV positivity was also observed in 23% (6/26) of benign samples. All biopsies of patients with positive serum samples were positive for EBV DNA.

Conclusion: EBV infection was found in sporadic NPC of South Indian origin, which confirms the etiological role of EBV in NPC. Detection of EBNA-1 in the serum and corresponding tissues of NPC patients suggests that the serum EBV DNA originates from NPC and also indicates the benefit of circulating viral DNA as an early marker in the diagnosis of NPC. Serum DNA-PCR methods can be extrapolated to follow-up studies involving tumor regression or to assess the response to various therapies.

Item ID: 33999
Item Type: Article (Refereed Research - C1)
Keywords: nasopharyngeal carcinoma, EBV, serum, PCR, biomarker
ISSN: 1465-3621
Funders: Indian Council of Medical Research
Date Deposited: 05 Aug 2015 00:36
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060404 Epigenetics (incl Genome Methylation and Epigenomics) @ 10%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111201 Cancer Cell Biology @ 70%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920102 Cancer and Related Disorders @ 100%
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