Therapeutic effect of curcumin supplementation in the modulation of NF-κB responsive genes in a collagen-induced arthritis rat model

Somasundaram, Shanmugam , Chakravarthi, Srikumar, Radhakrishnan, Ammu, Ramdas, Premdass, Nagaraja, Haleagrahara, and Kumari, Mangala (2014) Therapeutic effect of curcumin supplementation in the modulation of NF-κB responsive genes in a collagen-induced arthritis rat model. British Journal of Medicine & Medical Research, 4 (15). pp. 2940-2954.

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Abstract

Aims: To assess the therapeutic effect of curcumin supplementation in modulating the expression of NF-κB in the joints of collagen-induced arthritis (CIA) rats.

Place and Duration of Study: Department of Postgraduate Studies and Research, International Medical University, between July 2011 and May 2012.

Methodology: Arthritis was induced in each group of Dark Agouti (DA) rats, by intradermal injection with collagen emulsified in complete Freund’s adjuvant. Treatment groups which were induced with CIA were treated with: 500 mg/kg curcumin; 1000 mg/kg curcumin; 2000 mg/kg curcumin; 25 mg/kg aspirin. Combination treatment groups which were induced with CIA were treated with: 500 mg/kg curcumin and 25 mg/kg aspirin; 1000 mg/kg curcumin and 25 mg/kg aspirin; 2000 mg/kg curcumin and 25 mg/kg aspirin from day 25 to 38. Efficacy was assessed based on ability to reduce paw oedema, histopathological changes, NF-κB expression, serum tumour necrosis factor alpha (TNF-α), interleukin 1-beta (IL-1β) and gluthathione peroxidase (GPx) levels.

Results: Based on histopathological study, immunohistochemical scoring of NF-κB and ELISA analysis of TNF-α, IL-1β and GPx levels, our study found that curcumin given after arthritis in high doses, shows effects of healing and this results were comparable to positive control group, which is the arthritic group treated with 25 mg/kg aspirin. Curcumin given in combination with aspirin, showed better reduction in pathology in arthritic group compared to positive control group, especially with higher doses of curcumin.

Conclusion: Curcumin was effective in reducing inflammatory changes seen in CIA joints which were proved through histopathological, immunohistochemical and biochemical analysis, however only at high doses.

Item ID: 33857
Item Type: Article (Research - C1)
ISSN: 2231-0614
Keywords: curcumin; nuclear factor kappa B; collagen-induced arthritis; rheumatoid arthritis; gluthathione peroxidase; tumour necrosis factor alpha; interleukin 1-beta.
Additional Information:

© 2014 Somasundaram et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Funders: International Medical University (IMU), Malaysia
Projects and Grants: IMU Grant no. BS112010 - 95000, 2012 - 13
Date Deposited: 08 Jul 2014 23:59
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1111 Nutrition and Dietetics > 111103 Nutritional Physiology @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology @ 25%
11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110322 Rheumatology and Arthritis @ 25%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920116 Skeletal System and Disorders (incl. Arthritis) @ 50%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 25%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 25%
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