Simultaneous determination of coenzyme Q and plastoquinone redox states in the coral-Symbiodinium symbiosis during thermally induced bleaching

Lutz, Adrian, Motti, Cherie A., Freckelton, Mamie L., Van Oppen, Madeleine J.H., Miller, David J., and Dunlap, Walter C. (2014) Simultaneous determination of coenzyme Q and plastoquinone redox states in the coral-Symbiodinium symbiosis during thermally induced bleaching. Journal of Experimental Marine Biology and Ecology, 455. pp. 1-6.

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Abstract

Coenzyme Q (CoQ; ubiquinone) and plastoquinone (PQ) are metabolic electron carriers that, in their reduced state, are powerful antioxidants for cellular protection against oxidative damage. Although damage resulting from generation of reactive oxygen species (ROS) is strongly implicated in the initiation of symbiotic dysfunction that leads to coral bleaching, very little is known about the redox state of these two prenylquinone pools during the stress response. Here we describe a quantitative liquid chromatography-mass spectrometry (LC-MS) method that permits simultaneous measurement of the physiological redox state of both CoQ and PQin whole corals. The application of this method indicates that the CoQ and PQ pools in the coral-Symbiodinium symbiosis are maintained predominantly in their reduced (antioxidant) forms, and it is the coral CoQ redox state that is most affected by acute thermal stress.

Item ID: 33726
Item Type: Article (Research - C1)
ISSN: 0022-0981
Keywords: coenzyme Q, coral bleaching, LC-MS, mass spectrometry, oxidative stress, plastoquinone
Funders: Australian Institute of Marine Science (AIMS), AIMS@JCU
Date Deposited: 18 Jun 2014 09:44
FoR Codes: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060806 Animal Physiological Ecology @ 80%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060306 Evolutionary Impacts of Climate Change @ 20%
SEO Codes: 96 ENVIRONMENT > 9601 Air Quality > 960106 Urban and Industrial Air Quality @ 40%
96 ENVIRONMENT > 9603 Climate and Climate Change > 960399 Climate and Climate Change not elsewhere classified @ 60%
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