Fingerprinting genomic instability in oral submucous fibrosis

Teh, M.T., Tilakaratne, W.M., Chaplin, T., Young, B.D., Ariyawardana, A., Pitiyage, G., Lalli, A., Stewart, J.E., Hagi-Pavli, E., Cruchley, A., Waseem, A., and Fortune, F. (2008) Fingerprinting genomic instability in oral submucous fibrosis. Journal of Oral Pathology and Medicine, 37 (7). pp. 430-436.

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Abstract

Background: Oral submucous fibrosis (OSF) is a high-risk pre-cancerous condition where 7-13% of these patients develop head and neck squamous cell carcinoma (HNSCC). To date there is no cancer predictive markers for OSF patients. Genomic instability hallmarks early genetic events during malignant transformation causing loss of heterozygosity (LOH) and chromosomal copy number abnormality. However, to date there is no study on genomic instability in OSF. Although this condition is known as a high-risk pre-cancerous condition, there is no data regarding the genomic status of this disease in terms of genetic susceptibility to malignant transformation.

Methods: In this study, we investigated the existence of genetic signatures for carcinogenesis in OSF. We employed the high-resolution genome-wide Affymetrix Mapping single nucleotide polymorphism microarray technique to 'fingerprint' global genomic instability in the form of LOH in 15 patient-matched OSF-blood genomic DNA samples.

Results: This rapid high-resolution mapping technique has revealed for the first time that a small number of discrete hot-spot LOH loci appeared in 47-53% of the OSF tissues studied. Many of these LOH loci were previously identified regions of genomic instability associated with carcinogenesis of the HNSCC.

Conclusion: To our knowledge, this is the first evidence that genomic instability in the form of LOH is present in OSF. We hypothesize that the genomic instability detected in OSF may play an important role in malignant transformation. Further functional association studies on these putative genes may reveal potential predictive oral cancer markers for OSF patients.

Item ID: 33675
Item Type: Article (Research - C1)
ISSN: 1600-0714
Keywords: genomic instability; loss of heterozygosity; oral submucous fibrosis; single nucleotide polymorphic microarray
Funders: Institute of Dentistry, University of London (UL)
Projects and Grants: UL Central Research Fund grant AR ⁄ CRF ⁄ B
Date Deposited: 12 Nov 2014 02:36
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1105 Dentistry > 110505 Oral Medicine and Pathology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920113 Oro-Dental Disorders @ 100%
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