Impact of experimental hookworm infection on the human gut microbiota

Cantacessi, Cinzia, Giacomin, Paul, Croese, John, Zakrzewski, Martha, Sotillo, Javier, McCann, Leisa, Nolan, Matthew J., Mitreva, Makedonka, Krause, Lutz, and Loukas, Alex (2014) Impact of experimental hookworm infection on the human gut microbiota. Journal of Infectious Diseases, 210 (9). pp. 1431-1434.

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Abstract

The interactions between gastrointestinal parasitic helminths and commensal bacteria are likely to play a pivotal role in the establishment of host-parasite cross-talk, ultimately shaping the development of the intestinal immune system. However, little information is available on the impact of infections by gastrointestinal helminths on the bacterial communities inhabiting the human gut. We used 16S rRNA gene amplification and pyrosequencing to characterise, for the first time, the differences in composition and relative abundance of fecal microbial communities in human subjects prior to and following experimental infection with the blood-feeding intestinal hookworm, Necator americanus. Our data show that, while hookworm infection leads to a minor increase in microbial species richness, no detectable effect is observed on community structure, diversity or relative abundance of individual bacterial species.

Item ID: 33059
Item Type: Article (Research - C1)
ISSN: 1537-6613
Keywords: experimental infection; hookworms; parasitic helminths; 16S rRNA gene; pyrosequencing; chronic inflammatory disorders; microbial richness
Additional Information:

© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Funders: National Health and Medical Research Council of Australia (NHMRC), James Cook University (JCU), Isaac Newton Trust/Wellcome Trust ISSF/University of Cambridge Joint Research Grants Scheme
Projects and Grants: NHMRC grant 1052938, NHMRC grant 613718, NHMRC grant 037304, NHMRC grant 1020114, JCU FMHMS 2013 grants round
Date Deposited: 06 Aug 2014 02:05
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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