A comparative study: the impact of different lipid extraction methods on current microalgal lipid research

Li, Yan, Naghdi, Forough Ghasemi, Garg, Sourabh, Adarme-Vega, Tania Catalina, Thurecht, Kristofer J., Ghafor, Wael Abdul, Tannock, Simon, and Schenk, Peer M. (2014) A comparative study: the impact of different lipid extraction methods on current microalgal lipid research. Microbial Cell Factories, 13. 14. pp. 1-9.

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Abstract

Microalgae cells have the potential to rapidly accumulate lipids, such as triacylglycerides that contain fatty acids important for high value fatty acids (e.g., EPA and DHA) and/or biodiesel production. However, lipid extraction methods for microalgae cells are not well established, and there is currently no standard extraction method for the determination of the fatty acid content of microalgae. This has caused a few problems in microlagal biofuel research due to the bias derived from different extraction methods. Therefore, this study used several extraction methods for fatty acid analysis on marine microalga Tetraselmis sp. M8, aiming to assess the potential impact of different extractions on current microalgal lipid research. These methods included classical Bligh & Dyer lipid extraction, two other chemical extractions using different solvents and sonication, direct saponification and supercritical CO2 extraction. Soxhlet-based extraction was used to weigh out the importance of solvent polarity in the algal oil extraction. Coupled with GC/MS, a Thermogravimetric Analyser was used to improve the quantification of microalgal lipid extractions. Among these extractions, significant differences were observed in both, extract yield and fatty acid composition. The supercritical extraction technique stood out most for effective extraction of microalgal lipids, especially for long chain unsaturated fatty acids. The results highlight the necessity for comparative analyses of microalgae fatty acids and careful choice and validation of analytical methodology in microalgal lipid research.

Item ID: 32883
Item Type: Article (Research - C1)
ISSN: 1475-2859
Keywords: microalgal oil, fatty acid, extract yield, solvent polarity, supercritical CO2, lipid profile
Additional Information:

© 2014 Li et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Funders: Australian Research Council (ARC), Meat and Livestock Australia (MLA)
Date Deposited: 30 Apr 2014 09:55
FoR Codes: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 45%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 55%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8299 Other Plant Production and Plant Primary Products > 829999 Plant Production and Plant Primary Products not elsewhere classified @ 80%
85 ENERGY > 8505 Renewable Energy > 850501 Biofuel (Biomass) Energy @ 20%
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