GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile

Wangchuk, Phurpa, Keller, Paul A., Pyne, Stephen G., Taweechotipatr, Malai, and Kamchonwongpaisan, Sumalee (2013) GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile. Natural Product Communications, 8 (9). pp. 1305-1308.

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Abstract

We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

Item ID: 32825
Item Type: Article (Research - C1)
ISSN: 1555-9475
Keywords: Pleurospermum amabile, medicinal plant, essential oil, (E)-isomyristicin, antibacterial, antimalarial, multidrug resistant strain, cytotoxicity.
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Funders: Australian Endeavour Awards, Howard Hughes Medical Institute (HHMI)
Projects and Grants: HHMI International Research Scholar Grant
Date Deposited: 28 May 2014 04:15
FoR Codes: 03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030101 Analytical Spectrometry @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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