Antimalarial alkaloids from a Bhutanese traditional medicinal plant Corydalis dubia

Wangchuk, Phurpa, Keller, Paul A., Pyne, Stephen G., Willis, Anthony C., and Kamchonwongpaisan, Sumalee (2012) Antimalarial alkaloids from a Bhutanese traditional medicinal plant Corydalis dubia. Journal of Ethnopharmacology, 143 (1). pp. 310-313.

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Abstract

Ethnopharmacological relevance: Corydalis dubia is used in Bhutanese traditional medicine as a febrifuge and for treating infections in the blood, liver and bile which correlate to the signs and symptoms of malarial and microbial infections.

Aim of the study: To validate the ethnopharmacological uses of the plant and to discover potential new therapeutic drug leads.

Materials and methods: C. dubia was collected from Bhutan and the alkaloids were obtained using acid-base fractionation and separation by repeated column and preparative plate chromatography. The alkaloids were identified from analysis of their physiochemical and spectroscopic data and were tested for antiplasmodial, antimicrobial and cytotoxicity activities.

Results: A systematic extraction and isolation protocol yielded one new natural product, dubiamine, and seven known isoquinoline alkaloids, scoulerine, cheilanthifoline, protopine, capnoidine, bicuculline, corydecumbine and hydrastine. Among the four alkaloids tested, scoulerine showed the best antiplasmodial activity with IC50 values of 5.4 Antimalarial alkaloids Antimalarial alkaloids μM and 3.1 μM against the antifolate sensitive and the multidrug resistant P. falciparum strains: TM4/8.2 and K1CB1, respectively. None of the alkaloids tested showed significant antimicrobial or cytotoxicity activities.

Conclusions: The antiplasmodial test results, of the isolated alkaloid components, are commensurated with the ethnopharmacological uses of this plant.

Item ID: 32807
Item Type: Article (Refereed Research - C1)
Keywords: Bhutanese traditional medicine, medicinal plant, Corydalis dubia, antimalarial, Scoulerine
ISSN: 1872-7573
Date Deposited: 26 Jun 2014 04:55
FoR Codes: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030401 Biologically Active Molecules @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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