Antiplasmodial activity of atisinium chloride from the Bhutanese medicinal plant, Aconitum orochryseum

Wangchuk, Phurpa, Bremner, John B., Samten, , Skelton, Brian W., White, Allan H., Rattanajak, Roonglawan, and Kamchonwongpaisan, Sumalee (2010) Antiplasmodial activity of atisinium chloride from the Bhutanese medicinal plant, Aconitum orochryseum. Journal of Ethnopharmacology, 130 (3). pp. 559-562.

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Abstract

Ethnopharmacological relevance: The plant Aconitum orochryseum Stapf. (Ranunculaceae) is employed together with other plants in Bhutanese traditional medicine and is indicated for malaria-associated fever.

Aim of the study: To study the in vitro antiplasmodial activity of atisinium chloride, the major alkaloid from Aconitum orochryseum.

Materials and methods: Atisinium chloride was extracted and purified from aerial parts of Aconitum orochryseum and its structure and absolute configuration confirmed by single crystal X-ray crystallography. The crude methanol extract, crude alkaloid fraction, and atisinium chloride were tested for in vitro antiplasmodial activity against the malarial Plasmodium falciparum strains TM4/8.2 (TM4; wild type) and K1CB1 (K1; chloroquine and antifolate resistant).

Results: The diterpenoid alkaloid atisinium chloride was shown to have moderate antiplasmodial activities with IC50 values of 4 μM and 3.6 μM, respectively against the TM4 strain and the K1 strain of Plasmodium falciparum.

Conclusions: Our studies provide the first evidence in support of one of the indicated treatments with Aconitum orochryseum in Bhutanese traditional medicine. This alkaloid also represents a potential new antimalarial structural lead.

Item ID: 32780
Item Type: Article (Refereed Research - C1)
Keywords: Bhutanese traditional medicine; medicinal plant; Aconitum orochryseum; Ranunculaceae; atisinium chloride; antiplasmodial; crystal structure
ISSN: 1872-7573
Funders: Royal Government of Bhutan, DANIDA project
Date Deposited: 04 Jul 2014 04:20
FoR Codes: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030401 Biologically Active Molecules @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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