Virulence changes to Harveyi clade bacteria infected with bacteriophage from Vibrio owensii

Busico-Salcedo, Nancy, and Owens, Leigh (2013) Virulence changes to Harveyi clade bacteria infected with bacteriophage from Vibrio owensii. Indian Journal of Virology, 24 (2). pp. 180-187.

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Abstract

Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10^2 CFU ml^−1. This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml^−1. One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the up-regulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.

Item ID: 32540
Item Type: Article (Research - C1)
ISSN: 0974-0120
Keywords: Luminescent vibriosis, Vibrio owensii, Vibrio harveyi, Vibrio campbellii, bacteriophage VOB, virulence, Crustacea
Date Deposited: 15 Apr 2014 00:22
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology @ 25%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 25%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830105 Aquaculture Prawns @ 100%
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