Isolation and identification of Oedogonium species and strains for biomass applications

Lawton, Rebecca J., de Nys, Rocky, Skinner , Stephen, and Paul, Nicholas A. (2014) Isolation and identification of Oedogonium species and strains for biomass applications. PLoS ONE, 9 (3). e90223. pp. 1-9.

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DOI: 10.1371/journal.pone.0090223

View at Publisher Website: http://dx.doi.org/10.1371/journal.pone.0...

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Abstract

Freshwater macroalgae from the genus Oedogonium have recently been targeted for biomass applications; however, strains of Oedogonium for domestication have not yet been identified. Therefore, the objective of this study was to compare the performance of isolates of Oedogonium collected from multiple geographic locations under varying environmental conditions. We collected and identified wild-type isolates of Oedogonium from three geographic locations in Eastern Australia, then measured the growth of these isolates under a range of temperature treatments corresponding to ambient conditions in each geographic location. Our sampling identified 11 isolates of Oedogonium that could be successfully maintained under culture conditions. It was not possible to identify most isolates to species level using DNA barcoding techniques or taxonomic keys. However, there were considerable genetic and morphological differences between isolates, strongly supporting each being an identifiable species. Specific growth rates of species were high (>26% day^−1) under 7 of the 9 temperature treatments (average tested temperature range: 20.9–27.7°C). However, the variable growth rates of species under lower temperature treatments demonstrated that some were better able to tolerate lower temperatures. There was evidence for local adaptation under lower temperature treatments (winter conditions), but not under higher temperature treatments (summer conditions). The high growth rates we recorded across multiple temperature treatments for the majority of species confirm the suitability of this diverse genus for biomass applications and the domestication of Oedogonium.


Item ID:	32350
Item Type:	Article (Research - C1)
ISSN:	1932-6203
Additional Information:	© 2014 Lawton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funders:	Australian Government Cooperative Research Centre Scheme
Date Deposited:	14 Apr 2014 23:40
FoR Codes:	10 TECHNOLOGY > 1002 Environmental Biotechnology > 100203 Bioremediation @ 40% 10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 40% 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 20%
SEO Codes:	96 ENVIRONMENT > 9609 Land and Water Management > 960999 Land and Water Management of Environments not elsewhere classified @ 40% 85 ENERGY > 8505 Renewable Energy > 850599 Renewable Energy not elsewhere classified @ 30% 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8203 Industrial Crops > 820399 Industrial Crops not elsewhere classified @ 30%
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