Regulation of microRNA-1288 in colorectal cancer: altered expression and its clinicopathological significance

Gopalan, Vinod, Pillai, Suja, Ebrahimi, Faeza, Salajegheh, Ali, Lam, Tommy C., Le, Tran K., Langsford, Nicole, Ho, Yik-Hong, Smith, Robert A., and Lam, Alfred K.-Y. (2014) Regulation of microRNA-1288 in colorectal cancer: altered expression and its clinicopathological significance. Molecular Carcinogenesis, 53 (S1). E36-E44.

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Abstract

We aim to examine the miR-1288 expression in cancer cell lines and a large cohort of patients with colorectal cancer. Two colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. The miRNA expressions of miR-1288 were tested on these cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). An exogenous miR-1288 (mimic) was used to detect cell proliferation and cell cycle changes in SW480 using MTT calorimetric assay and flow cytometry, respectively. In addition, tissues from 122 patients with surgical resection of colorectum (82 adenocarcinomas, 20 adenomas, and 20 non-neoplastic tissues) were tested for miR-1288 expression by qRT-PCR. The colon cancer cell lines showed reduced expression of miR-1288 compared to normal colonic epithelial cell line. Over expression of miR-1288 in SW480 cell line showed increased cell proliferation and increased G2–M phase cells. In tissues, reduced miR-1288 expression was noted in majority of colorectal adenocarcinoma compared to colorectal adenoma and non-neoplastic tissues. Reduced or absent expression of miR-1288 was noted in 76% (n = 62/82) of the cancers. The expression levels of miR-1288 were higher in distal colorectal adenocarcinomas (P = 0.013) and in cancers of lower T staging (P  = 0.033). To conclude, alternation of miR-1288 expression is important in the progression of colorectal cancer. The differential regulation of miR-1288 was found to be related to cancer location and pathological staging in colorectal cancers.

Item ID: 31980
Item Type: Article (Research - C1)
ISSN: 1098-2744
Keywords: MicroRNA; miR-1288; colorectum; cell proliferation; cell cycle
Funders: Griffith University, James Cook University (JCU)
Projects and Grants: Collaborative Grants Scheme 2011-2012
Date Deposited: 21 May 2014 23:44
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1112 Oncology and Carcinogenesis > 111201 Cancer Cell Biology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920102 Cancer and Related Disorders @ 100%
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