Antibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processing

Saptarshi, Shruti R., Sharp, Michael F., Kamath, Sandip D., and Lopata, Andreas L. (2014) Antibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processing. Food Chemistry, 148. pp. 321-328.

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Abstract

The EF-hand calcium binding protein, parvalbumin, is a major fish allergen. Detection of this allergen is often difficult due to its structural diversity among various fish species. The aim of this study was to evaluate the cross-reactivity of parvalbumin in a comprehensive range of bony and cartilaginous fish, from the Asia-Pacific region, and conduct a molecular analysis of this highly allergenic protein. Using the monoclonal anti-parvalbumin antibody PARV-19, we demonstrated the presence of monomeric and oligomeric parvalbumin in all fish analysed, except for gummy shark a cartilaginous fish. Heat processing of this allergen greatly affected its antibody reactivity. While heating caused a reduction in antibody reactivity to multimeric forms of parvalbumins for most bony fish, a complete loss of reactivity was observed for cartilaginous fish. Molecular analysis demonstrated that parvalbumin cross-reactivity, among fish species, is due to the molecular phylogenetic association of this major fish allergen.

Item ID: 31768
Item Type: Article (Research - C1)
ISSN: 1873-7072
Keywords: parvalbumin, allergen, Asia-Pacific fish, seafood, IgE epitopes, monoclonal antibody
Funders: Australian Research Council (ARC)
Date Deposited: 26 Feb 2014 10:09
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics) @ 34%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110701 Allergy @ 33%
11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111705 Environmental and Occupational Health and Safety @ 33%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 50%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920120 Zoonoses @ 50%
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