Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)

Smith, Timothy M., York, Paul, Stanley, Annalise M., Macreadie, Peter, Keough, Michael J., Ross, D. Jeff, and Sherman, Craig D.H. (2013) Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae). Conservation Genetics Resources, 5 (3). pp. 607-610.

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Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia. We also tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri.

Item ID: 31737
Item Type: Article (Research - C1)
ISSN: 1877-7260
Keywords: Zostera nigricaulis, microsatellite markers, cross-amplification, genetic structure, seagrass
Funders: Victorian Department of Sustainability and Environment
Projects and Grants: Victorian Department of Sustainability and Environment (Seagrass resilience in Port Phillip Bay: developing better predictions of how seagrasses respond to environmental change)
Date Deposited: 26 Feb 2014 09:37
FoR Codes: 05 ENVIRONMENTAL SCIENCES > 0501 Ecological Applications > 050104 Landscape Ecology @ 30%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 40%
06 BIOLOGICAL SCIENCES > 0602 Ecology > 060207 Population Ecology @ 30%
SEO Codes: 96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960503 Ecosystem Assessment and Management of Coastal and Estuarine Environments @ 50%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960802 Coastal and Estuarine Flora, Fauna and Biodiversity @ 50%
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