Variation in amino acid content and its relationship to nitrogen content and growth rate in Ulva ohnoi (Chlorophyta)

Angell, Alex R., Mata, Leonardo, de Nys, Rocky, and Paul, Nicholas A. (2014) Variation in amino acid content and its relationship to nitrogen content and growth rate in Ulva ohnoi (Chlorophyta). Journal of Phycology, 50 (1). pp. 216-226.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1111/jpy.12154
 
27
5


Abstract

To evaluate the quantitative and qualitative changes in amino acids related to internal nitrogen content and growth rate of Ulva ohnoi, the supply of nitrogen to outdoor cultures of the seaweed was manipulated by simultaneously varying water nitrogen concentrations and renewal rate. Both internal nitrogen content and growth rate varied substantially, and the quantitative and qualitative changes in amino acids were described in the context of three internal nitrogen states: nitrogen-limited, metabolic, and luxury. The nitrogen limited state was defined by increases in all amino acids with increasing nitrogen content and growth up until 1.2% internal nitrogen. The metabolic nitrogen state was defined by increases in all amino acids with increasing internal nitrogen content up to 2.6%, with no increases in growth rate. Luxury state was defined by internal nitrogen content above 2.6%, which occurred only when nitrogen availability was high but growth rates were reduced. In this luxury circumstance, excess nitrogen was accumulated as free amino acids, in two phases. The first phase was distinguished by a small increase in the majority of amino acids up to ≈3.3% internal nitrogen, and the second by a large increase in glutamic acid, glutamine, and arginine up to 4.2% internal nitrogen. These results demonstrate that the relationship between internal nitrogen content and amino acid quality is dynamic but predictable, and could be used for the selective culture of seaweeds.

Item ID: 31013
Item Type: Article (Refereed Research - C1)
Keywords: algae; animal nutrition; arginine; critical nitrogen; glutamic acid; glutamine; luxury uptake; lysine; ethionine; nitrogen flux
ISSN: 1529-8817
Funders: Australian Government Cooperative Research Centre Scheme, Australian Renewable Energy Agency (ARENA)
Date Deposited: 28 Jan 2014 23:18
FoR Codes: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 30%
10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 40%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 30%
SEO Codes: 96 ENVIRONMENT > 9609 Land and Water Management > 960912 Urban and Industrial Water Management @ 50%
82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8203 Industrial Crops > 820399 Industrial Crops not elsewhere classified @ 50%
Downloads: Total: 5
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page