Panax notoginseng attenuates LPS-induced pro-inflammatory mediators in RAW264.7 cells

Rhule, Ava, Navarro, Severine, Smith, Jerry R., and Shepherd, David M. (2006) Panax notoginseng attenuates LPS-induced pro-inflammatory mediators in RAW264.7 cells. Journal of Ethnopharmacology, 106 (1). pp. 121-128.

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Herbals or dietary supplements are not regulated as drugs by the United States Food and Drug Administration (USFDA) although many may have associated therapeutic effects and toxicities. Therefore, the immunomodulatory effects of the herbal extract Panax notoginseng on cultured macrophages (RAW264.7 cells) were investigated to address potential therapeutic or toxic effects. Cells were stimulated with LPS (1 μg/ml) and treated with notoginseng at 5, 25 and 50 μg/ml. Notoginseng inhibited the LPS-induced production of TNF-α and IL-6 by the cultured macrophages in a concentration-dependent manner. The expression of COX-2 and IL-1β mRNA was also attenuated by notoginseng. TNF-α production was inhibited in samples treated with notoginseng 24 h before, or at the same time as LPS stimulation, but not in samples treated 8 h after LPS stimulation. Notoginseng reduced expression of the accessory molecules CD40 and CD86 on the RAW264.7 cells while CD14 and TLR4 expression remained unaffected. Furthermore, Rb1 and Rg1 ginsenosides also inhibited macrophage production of TNF-α, but to a lesser extent than did the whole notoginseng extract. Collectively, these results indicate that notoginseng inhibits LPS-induced activation of RAW264.7 macrophages and demonstrates that notoginseng possesses anti-inflammatory and immunosuppressive properties in vitro.

Item ID: 30993
Item Type: Article (Research - C1)
ISSN: 1872-7573
Keywords: Panax notoginseng; ginsenosides; RAW264.7; macrophages; inflammation
Projects and Grants: NSF-EPSCoR grant EPS00091995, NCRR grant P20 RR 017670
Date Deposited: 03 Jul 2014 23:54
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1104 Complementary and Alternative Medicine > 110404 Traditional Chinese Medicine and Treatments @ 10%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060103 Cell Development, Proliferation and Death @ 40%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 100%
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