Alterations in neuropeptide Y levels in the hypothalamus of the rat following treatment with methysergide

Dryden, S., McCarthy, H. D., Ware, M., Malabu, U., and Williams, G. (1993) Alterations in neuropeptide Y levels in the hypothalamus of the rat following treatment with methysergide. Clinical Science, 84 (Supplement No. 28). p. 44.

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Abstract

Methysergide (Meth) is a non selective 5-HT antrtgonist which stimulates feeding in rats. 5-HT has been suggested to induce satiety. Neuropeptide Y (NPY) is a 36 amino-acid peptide sbucturally related to pancreatic polypeptide, and one of the most abundant neuropeptide in the brain.

5-HT fibres project to the hypothalamus particularly the paravenmcular(PVN) and arcuatc (ARC) nuclei, sites of NPY action and synthesis respectively.

This study set out to examine the effect of acute and chronic adminisnation of methysergide on hypothalamic NPY. Male Wistar rats were used. In the acute experiment, rats were injected with either saline (0.9% n=8) or methysergide(l0mgkg n=8) and killed after four hours.

In the chronic experiment, rats were implanted with mini-osmotic pumps filled with either saline (n=8) or methysergide (lOmg/kg/day n=8). Rats were killed after seven days.

Food intake was greater in the methysergide treated rats after four hours compared with the saline treated rats p<0.01).

NPY levels were significantly increased in the PVN and ARC in the methysergide treated rats (PVN 61± 3.2 vs 47± 3 fmoUug protein p<O.OI. ARC 49± 4.2 vs 27± 3.2 pcO.01).

In the chronically mated rats food intake was raised by 13% over seven days (p<O.01).

Again NPY levels were significantly increased in the methysergide treated rats (PVN 66± 6.2 vs 47± 3.1 fmo/ug protein p=O.Ol. ARC 38.5 ± 3 vs 27.4± 3.2 p=0.02).

These results suggest that methysergide induced feeding is associated with changesin NPYergic activity in critical hypothalamic areas.

Item ID: 29861
Item Type: Article (Abstract)
Additional Information:

This special issue of Clinical Science contains communications of the Medical Research Society Meeting held on 5 and 6 November 1992.

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ISSN: 1470-8736
Date Deposited: 31 Mar 2014 07:42
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110306 Endocrinology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920104 Diabetes @ 100%
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