Summers, Phillip, and Pena, S.T. (2008) Seminal vesicle fluid reduces binding of porcine epididymal spermatozoa to oviduct explants. In: Proceedings of the 6th International Conference on Boar Semen Preservation (70) pp. 1387-1388. From: 6th International Conference on Boar Semen Preservation, 12-15 August 2007, Alliston, Canada.

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Abstract

The purpose was to determine if exposure of porcine epididymal sperm to seminal vesicle fluid (SVF) would influence the number of sperm bound to oviduct explants. Study design and methods: Testes and seminal vesicles were obtained from five 12 month-old Large White boars at slaughter. Sperm were collected from the rete testis, caput, corpus, and cauda epididymidis; an aliquot (50 μL) was suspended in 1 ml of SVF and incubated at 39 °C for either 1 or 30 min. For controls, sperm samples were incubated in Androhep medium (Petrunkina AM, et al. Reproduction 2001;121:889–96). After incubation, the sample was centrifuged at 600 × g for 10 min and sperm suspended in modified Androhep medium at 5 × 106 mL−1. Before and after incubation in SVF, the capacitation status was determined by the chlortetracycline assay and viability by eosin–nigrosin staining. Oviduct explants were prepared from the isthmus and ampulla from non-cycling 20-week-old gilts using procedures modified from previous reports (Petrunkina AM, et al. Reproduction 2001;121:889–96; Suarez SS, et al. Biol Reprod 1991;56:447–53). Oviduct explants were set up as six replicates for each sperm sample and after incubation with sperm for 15 min at 39 °C in 5% CO2 in air, the explants were fixed, stained and number of sperm bound in area of 1.25 mm2 counted. Data were analysed with ANOVA. Results: There was no significant difference in the number of sperm that bound to explants after incubation for 1 min in either Androhep medium or SVF, nor was there any difference in sperm viability (average 90% viability), but SVF significantly reduced the percentage of uncapacitated sperm (70% vs. 80%). After incubation for 30 min in SVF, fewer sperm (P ≤ 0.05) from the cauda and corpus, but not the caput or rete testis, bound to both isthmus and ampulla explants. The number that bound to the isthmus and ampulla explants was 6.23 ± 0.87 and 4.0 ± 0.47, respectively, whereas the controls were 13.13 ± 1.74 and 6.65 ± 0.68. There was no significant difference between the treatments for sperm viability, but the percentage of uncapacitated sperm declined significantly after incubation in SVF compared to the control (35% vs. 60% for caudal sperm). Conclusion: The induction of capacitation by exposure to SVF was responsible for the reduced binding of sperm from the corpus and cauda to oviduct explants. This effect was not evident in sperm from the caput or rete testis. It was suggested that seminal plasma increases the binding of caudal sperm to the isthmus (Petrunkina AM, et al. Reproduction 2001;121:889–96); perhaps in vivo, components of seminal plasma other than SVF counter the capacitating effect, and add molecules to sperm to facilitate binding to the oviductal epithelium.

Item ID:	29279
Item Type:	Conference Item (Abstract / Summary)
ISSN:	0093-691X
Date Deposited:	16 Sep 2013 05:41
FoR Codes:	07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070706 Veterinary Medicine @ 100%
SEO Codes:	83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830308 Pigs @ 100%
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