Immuno-fluorescence staining patterns of leukocyte subsets in the skin of taurine and indicine cattle

Constantinoiu, C.C., Jonsson, N.N., Jorgensen, W.K., Jackson, L.A., Piper, E.K., and Lew, A.E. (2013) Immuno-fluorescence staining patterns of leukocyte subsets in the skin of taurine and indicine cattle. Research in Veterinary Science, 95 (3). pp. 854-860.

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Abstract

The immuno-staining patterns of skin leukocytes were investigated in three breeds of cattle: Holstein-Friesian, Brahman and Santa Gertrudis of similar age before and after tick infestation. The antibodies specific for CD45 and CD45RO reacted with cells in the skin of all Holstein-Friesian cattle but did not react with cells in the skin of any Brahman cattle. The same antibodies reacted with cells from the skin of four (CD45) and seven (CD45RO) of twelve Santa Gertrudis cattle. The antibodies specific for T cell specific for MHC class II molecules labelled cells of mostly irregular shape, presumably dermal dendritic cells and/or acrophages and Langerhans cells. The antibody specific for granulocytes (mAb CH138) reacted with cells only in sections cut from skin with lesions. The antibody specific for CD25+ cells labelled regularly shaped cells that showed a wide range of intensities of staining.

Item ID: 29032
Item Type: Article (Research - C1)
ISSN: 1532-2661
Keywords: leukocyte, immuno-fluorescence, cattle, breed, skin
Funders: Cooperative Research Centre (CRC) for Beef Genetic Technologies, Australia
Date Deposited: 04 Nov 2013 00:43
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070705 Veterinary Immunology @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830301 Beef Cattle @ 100%
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