Lescano, H.G., Congdon, B.C., and Heather, N.W. (1994) Comparison of two potential methods of detecting Bactrocera tryoni (Diptera: Tephritidae) gamma-irradiated for quarantine purposes. Journal of Economic Entomology, 87. pp. 1256-1261.

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Abstract

We examined the use of both biochemical and anatomical techniques to detect irradiated larvae and pupae of the Tephritid fruit fly Bactrocera tryoni (Froggatt). Electrophoretic protein profiles for control and irradiated larvae were similar, irrespective of the age at which irradiation occurred. Protein profiles for control and irradiated pupae were also similar for the first 2d of pupation. A new protein band was detected in control pupae 3d after pupation began. This band was not observed in any irradiated pupae. However, because pupae cannot be aged morphologically, the absence of this protein band cannot be used to distinguish between irradiated and non-irradiated pupae of unknown age. Dissections of pupae indicated that the new protein band was not present because irradiated pupae died during the first days of pupation. No irradiated pupae survived to emergence. Electrophoretic techniques did not identify consistently either irradiated larvae or pupae of B. tryoni. The corrected size of the supraesophageal ganglion in irradiated mature larvae was significantly small than in untreated larvae, ragardless of the age at which irradiation occurred. This reduction was ~64% in samples treated as eggs (26h), first (72h), and second (96h) instars and 24% in third instars. A significant reduction in size of supraesophageal ganglion of mature third instars from all irradiation treatments suggests that this character may be successfully used to detect B. tryoni irradiated at quarantine dose rates. Irradiated samples had a significantly longer larval and pupation period, possibly because of the inability of irradiated larvae to feed normally. We also observed a significant increase in larval mortality with decreasing age at irradiation.

Item ID:	28829
Item Type:	Article (Research - C1)
ISSN:	1938-291X
Related URLs:	Publisher
Date Deposited:	11 Sep 2013 06:12
FoR Codes:	07 AGRICULTURAL AND VETERINARY SCIENCES > 0706 Horticultural Production > 070603 Horticultural Crop Protection (Pests, Diseases and Weeds) @ 100%
SEO Codes:	82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8202 Horticultural Crops > 820214 Tropical Fruit @ 100%
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