Polymerase chain reaction (PCR) primers for the amplification of five nuclear introns in vertebrates

Friesen, V.L., Congdon, B.C., Kidd, M.G., and Birt, T.P. (1999) Polymerase chain reaction (PCR) primers for the amplification of five nuclear introns in vertebrates. Molecular Ecology, 8 (12). pp. 2147-2149.

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Abstract

[Extract] Advancements in evolutionary genetics, as well as the conservation of biodiversity, increasingly require direct analyses of sequence variation in nuclear DNA. Recent studies indicate that nuclear introns have variabilities useful for both phylogenetics and population genetics (reviewed in Friesen 2000); however, use of introns is currently limited by the paucity of polymerase chain reaction (PCR) primers that have been demonstrated to have broad taxonomic utility (although several primers with less general or uncertain utilities have been published; reviewed in Friesen 2000). We have designed 30 general PCR primers for nuclear introns for vertebrates.

Item ID: 28817
Item Type: Article (Research - C1)
ISSN: 1365-294X
Date Deposited: 23 Aug 2013 00:15
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 80%
06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060302 Biogeography and Phylogeography @ 20%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960805 Flora, Fauna and Biodiversity at Regional or Larger Scales @ 100%
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