Schistosomiasis vaccine discovery using immunomics

Driguez, Patrick, Doolan, Denise L., Loukas, Alex, Felgner, Philip L., and McManus, Donald P. (2010) Schistosomiasis vaccine discovery using immunomics. Parasites & Vectors, 3 (1). 4. pp. 1-5.

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Abstract

The recent publication of the Schistosoma japonicum and S. mansoni genomes has expanded greatly the opportunities for post-genomic schistosomiasis vaccine research. Immunomics protein microarrays provide an excellent application of this new schistosome sequence information, having been utilised successfully for vaccine antigen discovery with a range of bacterial and viral pathogens, and malaria.Accordingly, we have designed and manufactured a Schistosoma immunomics protein microarray as a vaccine discovery tool. The microarray protein selection combined previously published data and in silico screening of available sequences for potential immunogens based on protein location, homology to known protective antigens, and high specificity to schistosome species. Following cloning, selected sequences were expressed cell-free and contact-printed onto nitrocellulose microarrays. The reactivity of microarray proteins with antisera from schistosomiasis-exposed/resistant animals or human patients can be measured with labelled secondary antibodies and a laser microarray scanner; highly reactive proteins can be further assessed as putative vaccines. This highly innovative technology has the potential to transform vaccine research for schistosomiasis and other parasitic diseases of humans and animals.

Item ID: 28637
Item Type: Article (Research - C1)
ISSN: 1756-3305
Keywords: antigen; schistosomiasis vaccine; unclassified drug; vaccine; antibody response; drug research; drug screening; genomics; high throughput screening; human; immunomic; malaria; molecular cloning; nonhuman; parasitosis; protein analysis; protein localization; protein microarray; protein structure; proteomics; review; Schistosoma japonicum; schistosomiasis; sequence homology; technology; Animalia; Bacteria (microorganisms); Schistosoma; Schistosoma japonicum; Schistosoma mansoni
Additional Information:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Date Deposited: 19 Aug 2013 05:28
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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