Schistosoma mansoni antigens alter the cytokine response in vitro during cutaneous leishmaniasis

Barbosa Bafica, Aline Michelle, Santos Cardoso, Luciana, Oliveria, Sergio Costa, Loukas, Alex, Varela, Giuseppe Tittoni, Oliveria, Ricardo Riccio, Bacellar, Olivia, Carvalho, Edgar Marcelino, and Araujo, Maria Ilma (2011) Schistosoma mansoni antigens alter the cytokine response in vitro during cutaneous leishmaniasis. Memorias do Instituto Oswaldo Cruz , 106 (7). pp. 856-863.

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Schistosoma mansoni infection or associated products are able to down-modulate the type 1 CD4+ T cell inflammatory response characteristic of autoimmune diseases. In this study, we evaluated how S. mansoni antigens altered the immune response that was induced by the soluble Leishmania antigen (SLA) from cutaneous leishmaniasis (CL) patients. Cytokines were measured from the supernatants of peripheral blood mononuclear cell cultures stimulated with SLA. This was performed using the sandwich enzyme linked immunosorbent assay technique in the presence or absence of S. mansoni recombinant antigens Sm29, SmTSP-2 and PIII. The addition of S. mansoni antigens to the cultures resulted in the reduction of interferon gamma (IFN-γ) levels in 37-50% of patients. Although to a lesser extent, the antigens were also able to decrease the production of tumour necrosis factor-alpha (TNF-α). We compared patients that either had or did not have reduction in IFN-γ and TNF-α production in cultures stimulated with SLA in the presence of S. mansoni antigens. We found that there was no significant difference in the levels of interleukin (IL)-10 and IL-5 in response to S. mansoni antigens between the groups. The antigens used in this study down-modulated the in vitro proinflammatory response induced by SLA in a group of CL patients through a currently undefined mechanism.

Item ID: 28555
Item Type: Article (Research - C1)
ISSN: 0074-0276
Keywords: cutaneous, leishmaniasis - S, mansoni, antigens, Sm29, SmTSP-2, PIII
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This paper is available under a Creative Commons, Attribution, Non-Commercial license. You may use this work for non-commercial purposes provided the original is correctly cited.

Date Deposited: 19 Aug 2013 23:43
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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