Changes in T-cell and monocyte phenotypes in vitro by Schistosoma mansoni antigens in cutaneous leishmaniasis patients

Barbosa Bafica, Aline Michelle, Santos Cardoso, Luciana, Oliveira, Sergio Costa, Loukas, Alex, Goes, Alfredo, Oliveira, Ricardo Riccio, Carvalho, Edgar M., and Araujo, Maria Ilma (2012) Changes in T-cell and monocyte phenotypes in vitro by Schistosoma mansoni antigens in cutaneous leishmaniasis patients. Journal of Parasitology Research, 2012. pp. 1-10.

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Abstract

High levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14⁺CD16⁺⁺) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14⁺⁺CD16⁻) and intermediate (CD14⁺⁺CD16⁺) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4⁺ T cells and they also expanded the frequency of CD4⁺CD25^highFoxp3⁺ T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.

Item ID: 28532
Item Type: Article (Refereed Research - C1)
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This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

ISSN: 2090-0023
Date Deposited: 19 Aug 2013 06:03
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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