C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches

Tozaki, Kana, Kimura, Junpei, Yasuda, Masahiro, Ryu, Nobuyuki, Nasu, Testuo, Pernthaner, Anton, and Hein, Wayne R. (2013) C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches. Journal of Veterinary Science, 14 (1). pp. 1-6.

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Abstract

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.

Item ID: 27676
Item Type: Article (Research - C1)
ISSN: 1976-555X
Keywords: bovine, follicle-associated epithelium, monoclonal antibody, Peyer's patch
Additional Information:

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Funders: Ministry of Education, Science, Sport and Culture, Japan, OVITA, New Zealand
Projects and Grants: 23405042
Date Deposited: 14 Jun 2013 04:54
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070705 Veterinary Immunology @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830310 Sheep - Meat @ 50%
83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830311 Sheep - Wool @ 50%
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