The serological diagnosis of melioidosis

Chantratita, Narisara, Lertmemongkolchai, Ganjana, Wuthiekanun, Vanaporn, and Norton, Robert (2012) The serological diagnosis of melioidosis. In: Ketheesan, Natkunam, (ed.) Melioidosis: a century of observation and research. Elsevier, The Netherlands, pp. 160-167.

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Abstract

Serology has a limited role in the diagnosis of melioidosis. Methods for serodiagnosis inc1ude the indirect haemagglutination assay (IHA), enzyme linked-immunosorbentassay (ELISA), immunofluorescent assay (IFA) and the immunochromatographic cassette test (ICT). The IHA is currently the most common test used but interpretation in endemic areas is difficult because of the presence of antibodies in apparently healthy individuals. The IHA assay is poorly standardised, with variation in Burkholderia pseudomallei strains, detection threshold and protocols for the polysaccharide antigen preparation. The ELISA and IFA IgG assays have similar sensitivities. The ELISA uses crude or purified aqueous protein extracts while the IFA uses bacterial cell wall components. The ELISA and IFA IgM assays are used as markers of disease activity. Cassette based ICT assays have been evaluated but lack sensitivity. More specific antigens are currently being investigated as candidates to facilitate early serological diagnosis. © 2012 Elsevier B.V. All rights reserved.

Item ID: 26517
Item Type: Book Chapter (Research - B1)
ISBN: 978-0-444-53479-8
Date Deposited: 19 Apr 2013 04:32
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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