Zygocotyle lunata: proteomic analysis of the adult stage

Sotillo, Javier, Valero, Luz M., Sanchez del Pino, Manuel M., Fried, Bernard, Esteban, J. Guillermo, Marcilla, Antonio, and Toledo, Rafael (2011) Zygocotyle lunata: proteomic analysis of the adult stage. Experimental Parasitology, 128 (2). pp. 133-137.

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Abstract

The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host–parasite relationships in infections with paramphistomes.

Item ID: 26350
Item Type: Article (Research - C1)
ISSN: 1090-2449
Keywords: proteomics, Zygocotyle, paramphistomid, trematoda; paramphistomes; Zygocotyle lunata; proteome; mass spectrometry
Date Deposited: 08 May 2013 00:15
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 60%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 40%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 60%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 20%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 20%
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