The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease

Marcilla, Antonio, Garg, Gagan, Bernal, Dolores, Ranganathan, Shoba, Forment, Javier, Ortiz, Javier, Muñoz-Antolí, Carla, Dominguez, M. Victoria, Pedrola, Laia, Martinez-Blanch, Juan, Sotillo, Javier, Trelis, Maria, Toledo, Rafael, and Esteban, J. Guillermo (2012) The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. PLoS Neglected Tropical Disease, 6 (2). e1513. pp. 1-9.

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Abstract

Background: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions.

Principal Findings: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified.

Conclusions: Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.

Item ID: 26337
Item Type: Article (Research - C1)
ISSN: 1935-2735
Keywords: transcriptomes, Strongyloides stercoralis, nematode
Additional Information:

© 2012 Marcilla et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: Fondo de Investigación Sanitaria (FIS), Spanish Ministry of Science and Innovation, FEDER, Conselleria d’Educació , Generalitat Valenciana
Date Deposited: 18 Apr 2013 01:52
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 60%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 20%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 20%
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