Defective endogenous retroviral elements in a canine lyphosarcoma cell line

Squires, R.A., Gorman, N.T., Padua, R.A., and Onions, D.E. (1989) Defective endogenous retroviral elements in a canine lyphosarcoma cell line. Journal of Veterinary Internal Medicine, 3 (2). p. 127.

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Abstract

A canine lymphosarcoma (LSA) cell has has been reported to produce particles with properties characteristic of mammalian retroviruses. However, it has proved difficult to reproduce this result. To search for retroviral proviral elements in this cell h e a bacteriophage Lambda genomic DNA library was made. The library was probed with a murine retrovirus (FMuLV) and hybridizing phage plaques were picked. DNA extracted from hybridizing phages was digested with restriction endonucleases and fragments were separated by agarose gel electrophoresis. Southern transfer and hybridization with FMuLV and baboon endogenous retrovirus (BaEV) revealed that 2 phage clones contained fragments which hybridized strongly with both FMuLV and BaEV. These fragments were subcloned into the plasmid vector "Bluescript" and sequenced by the dideoxy chain termination method.

DNA sequence analysis identified 2 distinct elements homologous with a variety of retroviruses.The larger fragment (2.5 kilobase pairs) contained 1.5kbp of a defective retroviral pol gene sharing 63% homology at the DNA level with AKV murine leukemia virus. The smaller fragment (0.45kb) consisted exclusively of retroviral pol gene almost identical to that in the large fragment, but with an additional 4Obp deletion. Sequencing on either side of these fragments is continuing.

In summary, defective endogenous retroviral elements have been found in a canine LSA cell he. The elements differ in their defects but appear otherwise to be closely related. Because of their defects, these elements could not be responsible for the production of intact retroviruses as reported in this cell line. Further study is in progress to determine whether other endogenous elements capable of producing intact retroviruses are present in this cell line. It is of interest to determine the prevalence of retroviral elements in normal canine DNA and to investigate whether they are of etiological significance in canine diseases.

Item ID: 26128
Item Type: Article (Abstract)
ISSN: 1939-1676
Date Deposited: 11 Jul 2013 01:34
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070706 Veterinary Medicine @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070712 Veterinary Virology @ 50%
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