Dinoflagellate bioluminescence: a key concept for studying organelle movement

Heimann, Kirsten, Klerks, Paul L., and Hasenstein, Karl H. (2013) Dinoflagellate bioluminescence: a key concept for studying organelle movement. In: Heimann, Kirsten, and Katsaros, Christos, (eds.) Advances in Algal Cell Biology. Marine and Freshwater Botany . Walter de Gruyter, Berlin, Germany, pp. 177-190.

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[Extract] Bioluminescence in Pyrocystis lunula depends on circadian movement of scintillons and chloroplasts between active and inactive locations. The involvement of the cytoskeleton in these transport processes was investigated using cytoskeleton-specific drugs. Chloroplast movements were visualized by chlorophyll autofluorescence and confocal laser scanning microscopy. Latrunculin B and Oryzalin prevented translocation of chloroplasts. Four hours after transition to the night phase, mechanically inducible bioluminescence was inhibited by Latrunculin B, the myosin inhibitor 2,3 butanedione monoxime (BDM), and Oryzalin in dose-dependent fashions. The actin stabilizer Jasplakinolide slightly enhanced bioluminescence but abolished Latrunculin B effects. The microtubule depolymerizer Colchicine had no effect on bioluminescence. These results suggest that F-actin and microtubules are involved in the movements of chloroplasts and scintillons. Although the tested drugs affected bioluminescence, further experiments are needed to determine if effects on bioluminescence were mediated by drug-induced failed translocations of scintillons or chloroplasts.

Item ID: 25697
Item Type: Book Chapter (Research - B1)
ISBN: 978-3-11-022960-8
Funders: US Department of Energy
Projects and Grants: DE-FG02-97- ER12220
Date Deposited: 24 May 2013 01:06
FoR Codes: 03 CHEMICAL SCIENCES > 0399 Other Chemical Sciences > 039901 Environmental Chemistry (incl Atmospheric Chemistry) @ 100%
SEO Codes: 96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960503 Ecosystem Assessment and Management of Coastal and Estuarine Environments @ 100%
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