Evidence for the classification of a crayfish pathogen as a member of the genus Coxiella

Cooper, A., Layton, R., Owens, L., Ketheesan, N., and Govan, B. (2007) Evidence for the classification of a crayfish pathogen as a member of the genus Coxiella. Letters in Applied Microbiology, 45 (5). pp. 558-563.

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Aims: The study aimed to provide characterization of a potential new species of Coxiella, identified following a series of outbreaks of disease in Australian native freshwater crayfish.

Methods and Results: PCR primers designed for amplification of Coxiella burnetii genes including 16S rDNA, com1 and sodB were used to amplify homologues in the Coxiella-like crayfish pathogen. Products were then cloned and sequenced. The organism demonstrated a high degree of sequence homology in the highly conserved 16S rDNA (96%) and sodB (99%) genes, as well as the Coxiella sp. specific com1 (100%) gene. Regions flanking the sodB coding sequence demonstrated homology to C. burnetii antioxidant AhpC/Tsa family protein and dihydrodipicolinate reductase gene.

Conclusions: The degree of homology between the genes selected and flanking regions suggested the two organisms were sufficiently closely related to belong to the same genus.

Significance and Impact of the Study: This study provided evidence for a potential new species in the currently monospecific genus Coxiella, with the only described member being C. burnetii, a category B biological warfare agent.

Item ID: 2352
Item Type: Article (Research - C1)
ISSN: 1472-765X
Keywords: 16S rDNA sequencing; Coxiella burnetii; genetic homology; phylogeny
Date Deposited: 10 Jun 2009 01:33
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%
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